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| Registros recuperados: 44 | |
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| Arnaud, Sophie; Goyard, Emmanuel; Prou, Jean; Vonau, Vincent; Bonhomme, Francois; Boudry, Pierre. |
| Exploitation of the pearl oyster Pinetada margaritifera, which has developed in French Polynesia over the last twenty years, is based mainly on the collection of natural spat. Recruitment is highly variable in space and time and so spat is very often transferred by the farmers between atolls or even between archipelagos. In previous studies, anonymous nuclear markers demonstrated that This development has coincided with a genetic homogenisation of the wild stocks since the 1980s. This suggests a high level of reproductive success on farms, which is likely to be due to the high density of animaIs when compared to the density in the wild. We used the same markers to show that the level of genetic variability observed on farms is not significantly different... |
| Tipo: Text |
Palavras-chave: French Polynesia; Genetic diversity; Spat; Pinctada margaritifera; Pearl oyster; Polynésie française; Diversité génétique; Naissain; Pinctada margaritifera; Huître perlière. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-3822.PDF |
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| Bahri Sfar, Lilia; Lemaire, Christophe; Chatain, Beatrice; Divanach, Pascal; Ben Hassine, Oum Kalthoum; Bonhomme, Francois. |
| A phylogeographic analysis based on 6 polymorphic microsatellites was performed on 15 sea bass samples. five from Western Mediterranean, seven from Eastern Mediterranean and three coming from French aquaculture stocks. Among the eastern samples, three did not cluster according to their geographic origin but rather with the occidental group. Furthermore. a somewhat lower allelic diversity was observed within these particular samples, indicating that they originated from a limited number of progenitors of foreign origin. Among the aquaculture stocks, only one revealed a significant reduction of genetic variability, indicating that these stocks are largely outbred and open to wild fishes. The fact that the use of occidental fingerlings to seed broodstocks in... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Farmed populations; Escapees; Molecular marker. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-410.pdf |
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| Desmarais, Erick; Belkhir, Khalid; Garza, John Carlos; Bonhomme, Francois. |
| Solitary LTR loci are the predominant form of LTR retrotransposons in most eukaryotic genomes. They originate from recombination between the two LTRs of an ancestral retrovirus and are therefore incapable of transposition. Despite this inactivity, they appear to have a substantial impact on the host genome. Here we use the murine RMER10 LTR family as an example to describe how such elements can reshape regions of the genome through multiple mutations on an evolutionary time scale. Specifically, we use phylogenetic analysis of multiple copies of RMER10 in rodent species, as well as comparisons of orthologous pairs in mouse and rat, to argue that insertions of members of this family have locally induced the emergence of tandem repeat loci as well as many... |
| Tipo: Text |
Palavras-chave: RMER10; Simple tandem repeats; LTR retrotransposons; Mouse genome. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2091.pdf |
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| Lemaire, Christophe; Versini, J; Bonhomme, Francois. |
| To investigate the origin and maintenance of the genetic discontinuity between Atlantic and Mediterranean populations of the common sea bass (Dicentrarchus labrax) we analysed the genetic variation at a fragment of mitochondrial cytochrome b sequence for 18 population samples. The result were also compared with new or previously published microsatellite data. Seven mitochondrial haplotypes and an average nucleotidic divergence of 0.02 between Atlantic and Mediterranean populations that matches a Pleistocene allopatric isolation were found. The frequency variation at the cytochrome b locus was many times greater between Atlantic and Mediterranean populations ((θ) over cap (C) = 0.67) than at microsatellite loci ((θ) over cap (N) = 0.02).... |
| Tipo: Text |
Palavras-chave: Reproductive isolation; Phylogeography; MtDNA; Microsatellites; Genetic transitions. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-776.pdf |
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| Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Bonhomme, Francois. |
| Oysters, like many marine species have a very high fecundity. Previous studies have shown that populations, from both hatcheries and the natural environment, have very low Ne/N ratios. These observations reveal high variation in reproductive success. In order to study individual reproductive success under controlled conditions, we used microsatellite markers to quantify parental contributions in in vitro crosses (5 males and 5 females) of Crassostrea gigas, the Pacific oyster. High polymorphism of the microsatellites (more that 50 alleles per locus) eased the parentage identifications. The results of a cross allowing gametic competition were compared with the results from a second cross where the gametes of the same parents were kept separate for each... |
| Tipo: Text |
Palavras-chave: Genetic; Hatchery; Microsatellite markers; Crassostrea gigas; Pacific oysters. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/acte-3457.pdf |
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| Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Kotoulas, G.; Bonhomme, Francois. |
| Oysters, like many other marine species, are characterized by very high fertilities. Natural or hatchery populations, often present very low Ne/N ratios (Hedgecock, 1994). It can be explained by very high variances of their breeding success (Li and Hedgecock, 1998), the effective population size being estimated through the temporal variance of the allelic frequencies in natural populations (Hedgecock et al., 1992) or, more directly, through the estimated number of founders in hatchery populations (Launey, 1998). This document is a presentation of the results of a number of experiments, realised in the scope of the European program "GENEPHYS" (FAIR 95-421), and using microsatellite markers to analyse the parental contributions in the flat oyster Crassostrea... |
| Tipo: Text |
Palavras-chave: Microsatellite; Succès reproducteur; Contributions parentales; Génétique; Crassostrea gigas; Huîtres. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/acte-3454.pdf |
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| Lapegue, Sylvie; Diaz-almela, E.; Launey, Sophie; Ledu, Christophe; Boudry, Pierre; Naciri-graven, Yamama; Bonhomme, Francois. |
| Natural populations of the European flat oyster, Ostrea edulis, have been harvested since Roman times. This species is distributed along the European Atlantic coasts, from Norway to Morocco, as well as the Mediterranean Sea and Black sea coasts. Previous studies, based on allozymes, have concluded that isolation by distance is taking place in the Mediterranean sea and the Atlantic ocean. Because of the presumed non neutrality of these markers, another approach was made to examine the genetic differentiation of this species using nuclear microsatellite and mitochondrial markers, both considered as neutral, and to compare the results to those observed with allozymes. |
| Tipo: Text |
Palavras-chave: Microsatellite markers; Ostrea edulis; Flat oyster; Genetic. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/acte-3497.pdf |
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| Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Camara, Mark D.; Lapegue, Sylvie. |
| Marine species with high fecundity and high early mortality may also have high variance in reproductive success among individuals due to stochastic factors, making successful reproduction a "sweepstakes." In some cases, the impact is sufficient to reduce the effective number of breeders in wild populations. We tested two predictions of the sweepstakes reproductive success hypothesis in a French Atlantic population of the European flat oyster, Ostrea edulis, by evaluating (1) whether individuals belonging to temporally discrete recruitment cohorts within a single reproductive season displayed reduced genetic variation relative to the entire adult population, and (2) whether these temporal cohorts of recruits were genetically differentiated from each other.... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-7399.pdf |
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| Boudry, Pierre; Launey, Sophie; Diaz-almela, E.; Naciri-graven, Yamama; Ledu, Christophe; Mira, Sara; Taris, Nicolas; Bonhomme, Francois; Lapegue, Sylvie. |
| Nowadays, PCR-based DNA markers allow the study of diversity and differentiation of marine organisms at different stages of their life cycle. Our study of European flat oyster (Ostrea edulis) populations has aimed to describe their spatial and temporal dynamics. First, we analyzed adults sampled over the natural range of the species. An Atlantic/Mediterranean differentiation pattern was observed. Results were congruent between allozymes, microsatellites and mitochondrial DNA and support an isolation by distance model. High variability was observed in the breadth of diversity between populations using a mitochondrial marker, this highlights the small effective population sizes in some locations. A 10-fold quantitative difference was observed in Fst... |
| Tipo: Text |
Palavras-chave: Larvae; Reproduction; Genetic; Ostrea edulis; European flat oyster; Population genetics. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/acte-3493.pdf |
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| Rolland, Jean-luc; Bonhomme, Francois; Lagardere, Francoise; Hassan, M; Guinand, Bruno. |
| Spatial and temporal population genetic structures of the common sole, Solea solea, were studied in Northeastern Atlantic and Mediterranean Sea populations, using three polymorphic exon-primed intron-crossing (EPIC) markers. Results demonstrated significant multilocus differentiation among Eastern Mediterranean and a group composed by Western Mediterranean and Atlantic populations (theta = 0.150, P < 0.001), but also suggested unrecorded genetic differentiation of the Adriatic Sea population. No pattern of isolation-by-distance was recorded across the range covered by sampling, from the Kattegat to the Aegean Sea. Conversely to genetically structured Mediterranean populations, Atlantic populations ranging from Denmark to Portugal could be considered as... |
| Tipo: Text |
Palavras-chave: Marine fish; Genetic structure; Introns; Common sole. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2400.pdf |
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| Diaz Almela, F; Boudry, Pierre; Launey, Sophie; Bonhomme, Francois; Lapegue, Sylvie. |
| The geographical structure of 15 natural populations of the flat oyster (Ostrea edulis L.) was assessed by single-strand conformation polymorphism (SSCP) of a 313-base-pair (bp) fragment of the mitochondrial 12S-rRNA gene. Fourteen haplotypes were observed, with one being dominant in the Mediterranean samples and another one in the Atlantic populations. The geographically extreme populations sampled in Norway and the Black Sea appeared differentiated by exhibiting the dominance of a third group of haplotypes. The results were compared to available microsatellite data at five loci. The Atlantic/Mediterranean differentiation pattern was qualitatively the same with both types of markers, confirming an isolation-by-distance pattern. The average mitochondrial... |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Polymorphism; Flat oyster; Population; Gene. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-680.pdf |
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| Hedgecock, Denis; Launey, Sophie; Pudovkin, A. I.; Naciri, Yamama; Lapegue, Sylvie; Bonhomme, Francois. |
| The great fecundity and very high larval mortality of most marine invertebrates and fish make possible substantial variance in the number of offspring contributed by adults to subsequent generations. The reproductive success of such organisms may thus resemble a sweepstakes lottery, in which a minority of progenitors succeeds in replacing an entire population, while the majority fails to procreate. One specific prediction of this hypothesis, that genetic diversity of newly settled cohorts should be less than that of the adult population, is tested in the present study. Microsatellite DNA markers were examined in naturally spawned juvenile European flat oysters Ostrea edulis (L.), collected over a 12-day period in 1993 from the western Mediterranean Sea,... |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2616.pdf |
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| Duranton, Maud; Allal, Francois; Fraisse, Christelle; Bierne, Nicolas; Bonhomme, Francois; Gagnaire, Pierre-alexandre. |
| Speciation is a complex process that leads to the progressive establishment of reproductive isolation barriers between diverging populations. Genome-wide comparisons between closely related species have revealed the existence of heterogeneous divergence patterns, dominated by genomic islands of increased divergence supposed to contain reproductive isolation loci. However, this divergence landscape only provides a static picture of the dynamic process of speciation, during which confounding mechanisms unrelated to speciation can interfere. Here we use haplotype-resolved whole-genome sequences to identify the mechanisms responsible for the formation of genomic islands between Atlantic and Mediterranean sea bass lineages. Local ancestry patterns show that... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00446/55750/57409.pdf |
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| Boudry, Pierre; Collet, Bertrand; Kotoulas, Georgios; Magoulas, Antonio; Hervouet, Veronique; Bonhomme, Francois; Gerard, Andre. |
| Highly polymorphic genetic markers can be useful tools for the analysis of parental contributions. The present paper reports 3 experiments using microsatellite markers to analyse parental contributions in controlled in vitro factorial crosses of the cupped oyster Crassostrea gigas. Parentage analysis was eased by the large polymorphism observed at the 3 loci studied. In a cross between 20 males and 20 females, a quarter of the progeny could be assigned to a given parent using a single microsatellite locus showing 28 alleles. In 3 crosses between 5 males and females, all the progeny could be assigned to a family by using 3 loci. In all cases, the combined genotyping at 2 loci allowed parentage to be detennined unambiguously. Despite the balanced gametic... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Microsatellites; Parental contributions; Crassostrea gigas; Microsatellites; Contributions parentales. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/acte-2739.pdf |
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| Brener-raffalli, Kelly; Clerissi, Camille; Vidal-dupiol, Jeremie; Adjeroud, Mehdi; Bonhomme, Francois; Pratlong, Marine; Aurelle, Didier; Mitta, Guillaume; Toulza, Eve. |
| Background: Although the term holobiont has been popularized in corals with the advent of the hologenome theory of evolution, the underlying concepts are still a matter of debate. Indeed, the relative contribution of host and environment and especially thermal regime in shaping the microbial communities should be examined carefully to evaluate the potential role of symbionts for holobiont adaptation in the context of global changes. We used the sessile, long-lived, symbiotic and environmentally sensitive reef-building coral Pocillopora damicornis to address these issues. Results: We sampled Pocillopora damicornis colonies corresponding to two different mitochondrial lineages in different geographic areas displaying different thermal regimes: Djibouti,... |
| Tipo: Text |
Palavras-chave: Coral holobiont; Microbiota; Bacterial communities; Symbiodinium assemblages; Thermal adaptation; Scleractinian corals; Coral reefs; Pocillopora damicornis. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00429/54076/55380.pdf |
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| Gagnaire, Pierre-alexandre; Broquet, Thomas; Aurelle, Didier; Viard, Frederique; Souissi, Ahmed; Bonhomme, Francois; Arnaud-haond, Sophie; Bierne, Nicolas. |
| Estimating the rate of exchange of individuals among populations is a central concern to evolutionary ecology and its applications to conservation and management. For instance, the efficiency of protected areas in sustaining locally endangered populations and ecosystems depends on reserve network connectivity. The population genetics theory offers a powerful framework for estimating dispersal distances and migration rates from molecular data. In the marine realm, however, decades of molecular studies have met limited success in inferring genetic connectivity, due to the frequent lack of spatial genetic structure in species exhibiting high fecundity and dispersal capabilities. This is especially true within biogeographic regions bounded by well-known... |
| Tipo: Text |
Palavras-chave: Connectivity; Gene flow; Marine conservation; Population genomics; Population structure. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00275/38592/37120.pdf |
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| Registros recuperados: 44 | |
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